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The Bandwagoner will yell at players who aren’t even on the team anymore

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Top of pageMETHODSThe primary polyclonal antibodies used for immunohistochemistry were raised in rabbits to the following human enzymes that participate in fatty acid very long chain acyl CoA dehydrogenase (VLCAD) (10), the MTP (9,11), medium chain acyl CoA dehydrogenase (MCAD; EC 1.3.99.3) (10), and short chain 3 hydroxyacyl CoA dehydrogenase (SCHAD; EC 1.1.1.35) (10). MTP antibodies labeled both the and of MTP. For reference, we used antibodies to other mitochondrial proteins: polyclonal antibodies to human 2 methyl 3 hydroxybutyryl CoA dehydrogenase (MHBD; EC 1.1.1.178) and mouse MAb to subunit I of the complex IV (cytochrome c oxidase; COX I; EC 1.9.3.1) of the respiratory chain (Molecular Probes Europe BV, Leiden, The Netherlands).

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